Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects
Identifieur interne : 000372 ( Main/Exploration ); précédent : 000371; suivant : 000373Guided bone regeneration using rhGDF‐5‐ and rhBMP‐2‐coated natural bone mineral in rat calvarial defects
Auteurs : Frank Schwarz [Allemagne] ; Daniel Ferrari [Allemagne] ; Martin Sager [Allemagne] ; Monika Herten [Allemagne] ; Brigitte Hartig [Allemagne] ; Jürgen Becker [Allemagne]Source :
- Clinical Oral Implants Research [ 0905-7161 ] ; 2009-11.
English descriptors
Abstract
Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.
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DOI: 10.1111/j.1600-0501.2009.01796.x
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type="city">Düsseldorf</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical Oral Implants Research</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2009-11">2009-11</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1219">1219</biblScope><biblScope unit="page" to="1230">1230</biblScope></imprint><idno type="ISSN">0905-7161</idno></series><idno type="istex">37E60E7AF3FC5FD1BDEDF1C07CA92B5F7464AEC7</idno><idno type="DOI">10.1111/j.1600-0501.2009.01796.x</idno><idno type="ArticleID">CLR1796</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-7161</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>animal study</term><term>guided bone regeneration</term><term>immunohistochemistry</term><term>rhBMP‐2</term><term>rhGDF‐5</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives: The aim of the present study was to assess the influence of either recombinant human growth and differentiation factor 5 (rhGDF‐5)‐ or recombinant human bone morphogenetic protein 2 (rhBMP‐2)‐coated natural bone mineral (NBM) on guided bone regeneration in a rat calvarial defect model. Material and methods: Two monocortical critical‐size calvarial defects (diameter 6 mm, depth 1.5 mm) were prepared in a total of 90 rats each (n=180 defects) and randomly allocated to the following groups: (1) NBM+collagen membrane (BG), (2) rhBMP‐2+NBM+BG, (3) rhGDF‐5+NBM+BG, (4) autogenous bone (AB)+BG, or (5) untreated control (C). At 1, 2, 4, 8, 16, and 24 weeks, dissected blocks were processed for histological [e.g. area (mm2) of mineralized tissue (MT)] and immunohistochemical (osteocalcin – OC, angiogenesis – TG) analysis. Results: At 2 weeks, both coated NBM groups exhibited the formation of a thin hard tissue bridge underneath the BG. All test groups revealed significantly higher mean MT values than the C group at 24 weeks. rhBMP‐2+NBM+BG‐treated defects revealed significantly higher mean MT values in comparison with the AB+BG (8 and 24 weeks), NBM+BG (2 and 4 weeks), and rhGDF‐5+NBM+BG (2, 16, and 24 weeks) groups, respectively. Immunoreactions to either OC or TG were comparable in all test groups. Conclusion: It was concluded that (i) all treatment procedures investigated supported bone regeneration at 24 weeks and (ii) rhBMP‐2 might have the potential to improve the outcome of healing, particularly during the early stages of healing.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Düsseldorf</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Düsseldorf</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Schwarz, Frank" sort="Schwarz, Frank" uniqKey="Schwarz F" first="Frank" last="Schwarz">Frank Schwarz</name></region><name sortKey="Becker, Jurgen" sort="Becker, Jurgen" uniqKey="Becker J" first="Jürgen" last="Becker">Jürgen Becker</name><name sortKey="Ferrari, Daniel" sort="Ferrari, Daniel" uniqKey="Ferrari D" first="Daniel" last="Ferrari">Daniel Ferrari</name><name sortKey="Hartig, Brigitte" sort="Hartig, Brigitte" uniqKey="Hartig B" first="Brigitte" last="Hartig">Brigitte Hartig</name><name sortKey="Herten, Monika" sort="Herten, Monika" uniqKey="Herten M" first="Monika" last="Herten">Monika Herten</name><name sortKey="Sager, Martin" sort="Sager, Martin" uniqKey="Sager M" first="Martin" last="Sager">Martin Sager</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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